ABSTRACT
Objective: To study the impact of Wolbachia surface protein (WSP) on reactive oxygen species (ROS) level inethanol (EtOH)-exposed HepG2 cells.Materials and Methods: Increase in ROS level was induced in HepG2 cells by subjecting HepG2 cells toEtOH exposure. Impact of WSP on ROS level was examined by staining of intracellular ROS in cells usingthe specific ROS-detecting dye 2ʹ, 7ʹ-dichlorodihydrofluorescein diacetate (H2DCFDA), followed by flowcytometric analysis.Results and Conclusion: Flow cytometry analysis using H2DCFDA-based staining study of ROS level inHepG2 cells revealed that EtOH caused oxidative stress in HepG2 cells by inducing production of high levelsof ROS. However, EtOH-induced increased ROS production in cells decreased with treatment of WSP.From the current study, we can culminate that WSP provides cytoprotective action against EtOH-inducedincreased ROS production and oxidative stress in HepG2 cells by decreasing ROS production. This will beof significance for the treatment of EtOH-related liver ailments. Thus, this article emphasizes that WSP withprotecting ability could be used as a powerful therapeutic drug to treat EtOH-related liver ailments.